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KMID : 1094720130180051031
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Biotechnology and Bioprocess Engineering
2013 Volume.18 No. 5 p.1031 ~ p.1037
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Preparation and evaluation of a peptide nucleic acid gene chip system associated with surface plasmon resonance
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Ou Qingye
He Jian’an
Liu Chunqiao
Shi Lei
Zhao Chunzhong
Xu Yungqing
Gu Dayong
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Abstract
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The aim of this study was to construct a gene chip system based on a surface plasmon resonance technique, where peptide nucleic acid (PNA) oligomers are used as probes. Since the self-assembled monolayer (SAM) technology offers good control at the molecular level, we prepared 2D surface chemistry via SAM for probe attachments. PNA, which was designed according to the bioinformatics, was immobilized on the SAM-modified chip, and subsequently, relevant parameters of the experiment were ensured and optimized. Our results suggest that the ion strength and pH value of the buffer solution do not play significant roles in PNA or its complementary strand hybridization. The PNA probe binds to its complementary nucleic acid strand with a higher sensitivity and specificity compared to those of a traditional DNA probe. The PNA probe combined with surface plasmon resonance (SPR) technology has the benefits of being a label-free and in-real time monitor, as well as having improved hybridization and stability efficiency, which highlight the PNA gene chip detection system as a promising biosensor for clinical applications.
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KEYWORD
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surface plasmon resonance, peptide nucleic acid, self-assembled monolayer, gene chip, biosensor
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